Abstract

PONCE DE LEON, Patricia; FORESTO, Patricia  and  VALVERDE, Juana. Study of hyaluronic acid capture by Ascaris lumbricoides. Acta bioquím. clín. latinoam. [online]. 2009, vol.43, n.4, pp.625-630. ISSN 0325-2957.

Hyaluronan Acid (HA) has important functions in immunity. Previous experiences have shown that A. lumbricoides's extracts of adult specimens and larval concentrates have hyaluronan binding capacity. The aim of this study was to analyse the HA capture by this helminth. Three extracts of adult specimens ([AE]_B,C,D) and 3 larval concentrates ([ALLC₁]: 1100 to 1200 larvae/ mL; [ALLC₂]: 400 to 600 larvae/ mL and [ALLC₃]: 100 to 200 larvae/ mL) were studied. The modified test of serum soluble CD44 Detection by Aggregation Inhibition was used. CexpAdhE_AH was defined as the quotient between erythrocytes adhered by the HA in presence and in absence of the parasite, and IexpCP_AH was defined as the amount of erythrocytes that stopped adhering due to the HA capture by the parasite in relation to the total number of erythrocytes. The results showed significant differences in CexpAdhE_AH and IexpCP_AH as the result of larvae concentration and of the extracts. The arithmetic means of CexpAdhE_AH and IexpCP_AH for the larvae were 0.636 and 0.21 ([ALLC₁]); 0.819 and 0.068 ([ALLC₂]); 0.97 and 0.013 ([ALLC₃]). Medians of CexpAdhE_AH and IexpCP_AH for the extracts were [EA]_C:0.275 and 0.4; [EA]_B: 0.20 and 0.43; [EA]_D: 0.075 and 0.495. From the experience, it could be assumed that the parasite can capture the HA to interfere in the host's immune response.

Keywords : Ascaris lumbricoides; Hyaluronic acid; Capture.

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