Abstract

ZARATE, Mariela Soledad et al. Development and assessment of a sample for quality control in Microbiology. Acta bioquím. clín. latinoam. [online]. 2007, vol.41, n.1, pp.57-61. ISSN 0325-2957.

The microbiology laboratory requires an appropriate quality control in each stage of the sample procedure. To date, interlaboratory studies that evaluate the initial stages of microbiologic analysis procedures have not been reported. The development of a lyophilized sample for isolation control and identification of microorganisms (mo) was performed with that purpose. For the sample's elaboration a peritoneal dialysis liquid was inoculated with gram negative bacilli (GNB) and gram positive cocci (GPC). The samples were divided into 2 mL vials and lyophilized according to the protocol provided by ProgBA. Thirty four laboratories received the sample with instructions for its handling and results report. The rate of response was of 44%. The percentage of success in microscopic evaluation was 100% and 79% for BGN and CGP respectively. Seventy per cent of the laboratories succeeded in isolating and identifying both microorganisms correctly; 100% of them identified the GNB. Considering an 80% of agreement in mo recovery a validity criterion, the results obtained were acceptable for GNB. This protocol could be used for the preparation of an interlaboratory quality control scheme on a greater scale.

Keywords : control quality; microbiology.

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