Abstract

MONTALVO-CORRAL, Maricela et al. Standardization of a molecular detection method of highly pathogenic avian influenza virus (H5N1). Acta bioquím. clín. latinoam. [online]. 2009, vol.43, n.1, pp.49-52. ISSN 0325-2957.

Highly pathogenic avian influenza virus (HPAI) H5N1 is a global threat due to its zoonotic and pandemic potential. Then, concern arises and the need to have early detection tools to minimize the impact on human and animal health. In this work, a molecular detection method was implemented to detect matrix (M), hemagglutinin (H5) and neuraminidase (N1) genes of HPAI avian influenza virus H5N1, based on real time RT-PCR (RRT-PCR). Positive controls were constructed from reference RNA viral A/Vietnam/1203/2004 (H5N1), cloned into plasmidic vectors and sequenced. Assay detection sensitivity was assessed with standard curves for each gene. Assay sensitivity was 10² DNA copies/μl in all cases. Curves showed amplification efficiency higher than 90% and R²>0.99. This method could be useful for bird monitoring campaigns and as a screening procedure for clinical samples.

Keywords : Avian influenza; Detection; Real time reverse transcription-polymerase chain reaction.

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