Abstract

PONCE DE LEON, Patricia; FORESTO, Patricia    and  VALVERDE, Juana  . Ascaris lumbricoides: Study of kinetics of hyaluronic acid capture. Acta bioquím. clín. latinoam. [online]. 2010, vol.44, n.1, pp.53-62. ISSN 0325-2957.

Hyaluronic acid (HA) particípales in the immune response because it is a constituent of natural mechanical barriers, an inflammation stimulator and a ligand for receptors involved in immunity. Previous experiences have shown that adult specimens and larval concéntrales oí A. lumbricoides have hyaluronan binding capacity. The aim ofthis work was to study the kinetics ofhyaluronic acid capture by this parasite. Work was carried out with an extract of adult specimen and a larval concéntrate. The modlfled test of serum soluble CD44 Detectlon by Aggregatlon Inhlbltlon was used. Kinetics hyaluronlc acid capture by the nematode was assessed by varylng the contad time between HA and parasite, from an initial time to 90 minutes, with 10-minute intervals. C_expAdhE_HA ana lexpCP_HA were calculated for all the times and they were analysed by Wilcoxon and Friedman Tests The results showed that at initial time of contad, the adult paraslte and the larvae had captured a small amount of HA. The major capture was made at 10 minutes and it was maintained for 50 minutes without significad differences. From that time there was a slight increase in capture and it remaíned so untíi the end of the experiment without considerable variations. This experience demonstrates that at 10 minutes of in vitro contad, A. lumbricoides captures most of the HA, whích suggests that in vivo, the parasite would be able to capture HA quickly for the purpose of modulating the immune response.

Keywords : Ascaris lumbricoides; Hyaluronic acid; Kinetics; Capture.

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