Abstract

PONCE DE LEON, Patricia; DI VITA, Santiago; BIONDI, Claudia  and  VALVERDE, Juana. Sialic acid capture by Ascaris lumbricoides larvae during in vivo incubation. Acta bioquím. clín. latinoam. [online]. 2011, vol.45, n.3, pp.455-461. ISSN 0325-2957.

Erythrocyte aggregation affects microcirculation and its study is important in vascular diseases. It was communicated that Ascaris lumbricoides can capture erythrocyte sialic acid (SA) and alter the anionic charge on the red cell. The aim of this work was to study SA capture by A. lumbricoides larvae incubated in vivo with erythrocytes. Work was performed with concentrated larvae ([ALLC]) incubated in 4 tubes with phosphate buffer and antibiotics. Group O erythrocytes were added in two Tubes. The remaining were Controls. They were incubated at 37 °C (5% CO₂) for 24 and 28 hours. The larvae were separated, collected, concentrated and counted microscopically (larvae/mL: [ALLC]1:1500-1700; [ALLC]3: 1600-1800; [ALLC]2 and 4: 200- 400). Aggregation Inhibition by Polybrene (AIP) and Blue Alcian (BA) techniques were used. AIP showed a significant difference between Polybrene Titers diluted with physiological solution and with [ALLC]1 and 2, which were incubated with erythrocytes 24 and 48 hours respectively. There was no change in the Title of the corresponding Controls. BA performed in [ALLC]1 and [ALLC]3 determined CapSAC = 6.65% ± 0.36 and SAC_[CLAL]3 %= 0.67% ± 0.36. The experience showed SA capture by larvae incubated in vivo with erythrocytes and it suggested that they have no intrinsic SA. SA kidnapping during the larval migration could be important in parasite-host interaction.

Keywords : Capture; Sialic acid; Larvae; Ascaris lumbricoides.

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