Abstract

PEANO, Natalia Andrea; COLLINO, César Juan Gerardo; MERCADO, Luis Gabriel  and  VETTORAZZI, Lorena. Efficiency of human umbilical cord as substrate or detection of antiemdomysium antibodies. Acta bioquím. clín. latinoam. [online]. 2012, vol.46, n.3, pp.353-357. ISSN 0325-2957.

The antiendomysium antibodies (EMA) are directed toward antigens of connective tissue that surrounds the smooth muscle fibers. The aim of this study was to evaluate the efficiency of human umbilical cord (HUC) as substrate to detect EMA by indirect immunofluorescence and to compare it with one of the commercially available methodologies which use monkey esophagus as substrate. Serum samples obtained from 100 patients with celiac disease diagnosis and 50 healthy controls with normal intestinal mucosa were evaluated. Patients were treated at the Hospital Privado Centro Médico de Córdoba over a period of time between 2006 and 2009. The results showed an "almost perfect" concordance between both methods. The calculated sensitivity and specificity for HUC was 98% (93-99%) and 100% (93-100%) respectively, with an efficiency of 99%. This results indicate that the use of HUC as substrate to evaluate the presence of EMA is reliable and effective for the detection of patients with untreated celiac disease.

Keywords : Antiendomysium antibodies; Monkey esophagus; Human umbilical cord; Serology; Celiac disease.

        · abstract in Spanish | Portuguese     · text in Spanish     · Spanish ( pdf )