Abstract

CORDERO PEREZ, Paula et al. Comparative study of two methods in the determination of autoantibodies in autoimmune liver diseases. Acta bioquím. clín. latinoam. [online]. 2014, vol.48, n.4, pp.403-408. ISSN 0325-2957.

Autoimmune hepatitis is a hepatocellular inflammation which is characterized by different circulating autoantibodies. In this paper, the correlation between the results obtained by ELISA and indirect immunofluorescence technique  for the determination of autoantibodies in autoimmune hepatitis was evaluated. One hundred and twenty-three patients with autoimmune hepatitis, 91 (74%) female and 32 (26%) male, over 18 years were included. In these patients, a comparative study between ELISA and indirect immunofluorescence detection of the antinuclear antibodies (78 patients), anti-mitochondrial antibodies (84 patients) and anti-liver kidney microsome (85 patients) was performed. According to the kappa value obtained, it was found that for anti-liver kidney microsome the level of agreement was very good (k=1.0, p<0.001) for antinuclear antibody, the level of concordance was weak but significant (k=0.37, p<0.001,) meanwhile antimitochondrial antibody level of concordance was poor (k=0.05, p<0.476).The determination of the antibody anti-liver kidney microsome was the test with greater sensitivity, specificity and concordance between the two techniques discussed and it was established that the ELISA technique for antimitochondrial antibody is only in concordance with the indirect immunofluorescence in patients with high titers.

Keywords : Autoimmune hepatitis; Autoantibodies; Antinuclear antibodies; Antimitochondrial antibodies; Anti-liver kidney microsome; Immuno sorbent assay enzyme-linked; Indirect immunofluorescence.

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