Abstract

JACOME MANCHENO, Sandy Carolina; LOPEZ MOLINA, Melba Noemí; GABELA BAQUERO, Martha Elizabeth  and  CHIRIBOGA-PONCE, Rosa F. Titulación de hemolisinas en concentrados plaquetarios obtenidos a partir de buffy coat de donantes voluntarios de sangre de grupo “O” Rh D positivo. Acta bioquím. clín. latinoam. [online]. 2020, vol.54, n.3, pp.285-292. ISSN 0325-2957.

The possible adverse effects that occur in incompatible ABO transfusions are a latent risk in the use of group O platelet concentrates, so the titration of anti-A/B hemolysins is one of the strategies for its prevention. The objective of this study was to determine the frequency of hemolysins titers IgG and IgM anti-A/B isotypes in blood donours. It was a simple randomized descriptive cross-sectional study with a sample size of 308 samples. The methodology was applied in tube, saline gel and anti-IgG anti-immunoglobulin and by means of serial solutions the title was verified. Additionally, a survey was conducted on the possible risk factors for the increase in securities. Descriptive statistics were used through the application of the SPSS version 22.0 software and the relationship between independent variables through the Chi-square statistical analysis and the kappa index was applied to match the visual readings of the gel cards. The existence of IgG and IgM anti-A/B isotype hemolysins of titers greater than 1/64 was determined. There was a statistically significant relationship between pregnancies and anti-A/B IgG titres>1/128; and the increase in IgM isotype hemolysins and probiotic intake. The results demonstrate the need to implement hemolysin titration prior to transfusion of non-isogroup platelet concentrates, so a risk-benefit investigation and follow-up of patients with transfusions of ABO incompatible platelet concentrates is recommended.

Keywords : Hemolysins; Titration; Anti A/B hemolysins; IgG-IgM isotypes; ABO incompatibility.

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