Abstract

VISCIARELLI, Elena Concepción et al. Dinámica de la evolución de las formas de Blastocystis spp. en un medio de cultivo simple. Acta bioquím. clín. latinoam. [online]. 2022, vol.56, n.3, pp.314-321. ISSN 0325-2957.

Blastocystis spp. is a very frequent parasite in human fecal matter, but the polymorphic nature and the number of Blastocystis in a sample can complicate its detection by microscopy. The objective of the present work was to describe the dynamics of Blastocystis morphotypes in the short term in a simple culture medium and to determine its applicability to use it as a complement to coproparasitological analysis and for morphological, biochemical and molecular studies of the parasite. Ten stool samples with Blastocystis were cultured in an adapted Pavlova medium and examined during 6 days by microscopy to record the forms and the count. The development was regular and abundant and the shapes were of variable sizes and clearly identifiable. The tested culture could be used for the detection of Blastocystis when microscopic diagnosis is dubious, for studies of diagnostic sensitivity and specificity or when it is necessary to increase the load to perform other studies.

Keywords : Blastocystis spp; ; Culture; Blastocystis forms; Pavlova’s medium; Coproparasitological analysis.

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