Abstract

TOGNERI, Ana María et al. Utilidad de la implementación de un método molecular automatizado en el diagnóstico de agentes causales de infecciones de transmisión sexual. Acta bioquím. clín. latinoam. [online]. 2023, vol.57, n.3, pp.297-302. ISSN 0325-2957.

The results obtained in the first months after the implementation of the diagnosis of causative agents of sexually transmitted infections (STIs) by real- time PCR in an automated system are presented. Forty-six endocervical samples and 39 male urethral samples were studied by fresh microscopic examination, Gram staining, blood agar culture, Thayer Martin agar, miniaturised galleries to investigate Ureaplasma spp. and Mycoplasma hominis (Mycoplasma IST3, bioMérieux, France), and multiplex PCR for Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis (BDMaxTM System, Becton Dickinson, USA). Using multiplex PCR, an agent linked to STIs was detected in 48.7% of the urethral samples and in 21.7% of the endocervical samples, in addition to 5 cases of coinfection. A total of 18.8% of the infections were diagnosed only by PCR. These data show that PCR optimised the diagnosis of STIs in people of both sexes.

Keywords : Sexually transmitted infections; Multiple PCR; Coinfection; Diagnosis.

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