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Revista argentina de microbiología
Print version ISSN 0325-7541
Abstract
DI CONZA, José A; MOLLERACH, Marta E; GUTKIND, Gabriel O and AYALA, Juan A. Dos aislamientos de Salmonella Infantis multirresistentes se comportan como hipoinvasivos pero con elevada proliferación intracelular. Rev. argent. microbiol. [online]. 2012, vol.44, n.2, pp.69-74. ISSN 0325-7541.
Two multidrug-resistant Salmonella Infantis isolates behave like hypo-invasive strains but have high intracellular proliferation. In this work, plasmid-encoded virulence factors in two Salmonella Infantis isolates carrying multiresistance plasmids were investigated. In addition, their invasion and proliferative ability in non-phagocytic cells was studied. None of them showed the typical determinants of virulence plasmids (spv operon). The invasion assays of S. Infantis isolates on eukaryotic cells showed a decreased ability to Invade but they remained and proliferated In the cytoplasm regardless of having used a permissive (HeLa) or non-permissive (NRK) cell line. Finally, there was no microscopic evidence suggesting a bactericidal effect of these eukaryotic cell lines on the Isolates tested.
Keywords : Salmonella Infantis; Multiresistance; Invasion ability; Intracellular proliferation.