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Revista argentina de microbiología
Print version ISSN 0325-7541On-line version ISSN 1851-7617
Abstract
YAO, Shulin et al. Control of agitation and aeration rates in the production of surfactin in foam overflowing fed-batch culture with industrial fermentation. Rev. argent. microbiol. [online]. 2015, vol.47, n.4, pp.344-349. ISSN 0325-7541. http://dx.doi.org/10.1016/j.ram.2015.09.003.
Bacillus amyloliquefaciens fmb50 produces a high yield of surfactin, a lipopeptide-type biosurfactant that has been widely studied and has potential applications in many fields. A foam overflowing culture has been successfully used in the combined production-enrichment fermentation of surfactin. In this study, the agitation and aeration rates were found to have relationships with foam formation and surfactin enrichment. A maximum surfactin concentration of 4.7 g/l of foam was obtained after 21 h of culture with an agitation rate of 150 rpm and an aeration rate of 1 vvm in fed-batch culture. By controlling the foam overflow rate (fout) of a fed-batch culture, surfactin concentration in the foam was continuously maintained above 4 g/l.
Keywords : Surfactin; Agitation rate; Aeration rate; Foam overflowing; Fed-batch culture; Industrial fermentation.