Abstract

CRUZ FACUNDO, Itzel Maralhi et al. Enterotoxigenic profiles and submerged and interface biofilms in Bacillus cereus group isolates from foods. Rev. argent. microbiol. [online]. 2023, vol.55, n.3, pp.11-11. ISSN 0325-7541.  http://dx.doi.org/10.1016vj.ram.2023.01.007.

Biofilm formation by Bacillus cereus strains is now recognized as a systematic contaminaron mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dex-trose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group iso-lated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.

Keywords : Bacillus cereus; Enterotoxins; Biofilm; Food.

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