Abstract

LU, X. Y.; JIA, Z. H.; LI, J. W.  and  ZHANG, F. C.. Novel optical inmunoassay based on macroporous silicon waveguide for determining hidroxysafflor yellow. Lat. Am. appl. res. [online]. 2012, vol.42, n.1, pp.61-64. ISSN 0327-0793.

Porous silicon (PSi) has attracted much attention for biosensing due to its large surface area and easy preparation. In this paper, an optical immunoassay method based on a macro-PSi silicon resonant waveguide, with pore size much larger than that of large molecules such as bovine serum albumin, has been developed for the detection of the antigen-antibody reaction between hydroxysafflor yellow A (HSYA, i.e., the component of Carthamus tinctorius L.) and the specificity of the polyclonal anti-HSYA antibodies. HSYA antibodies were immobilized into the macro-PSi silicon waveguide using standard amino-silane and glutaraldehyde chemistry. The waveguide resonance angle was increased by binding HSYA onto the immobilized antibodies. The label-free immunosensor is simple and exhibits high sensitivity to HSYA. Therefore, this research is expected to have applications for quick and accurate determination of HSYAand can also be used for various immunoassays with other antigens..

Keywords : Porous Silicon; Waveguide; Label-Free Biosensor; HydroxySafflor Yellow A..

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