Abstract

FUENTEALBA, Nadia Analía et al. Producción de la glicoproteína C recombinante de alfaherpesvirus equino 1 y evaluación de la respuesta inmune inducida en ratones. Analecta vet. [online]. 2021, vol.41, n.1, pp.2-2. ISSN 1514-2590.  http://dx.doi.org/https://doi.org/10.24215/15142590e053.

Equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortions, perinatal death and neurological disorders in horses. Envelope glycoproteins are the most immunodominant antigens in infected animals. Natural infection and available vaccines provide partial and short-term protection against reinfection. In this study, EHV-1 glycoprotein C was expressed with a recombinant baculovirus and the ability to induce protective immunity in BALB/c mice challenged with the virus was analyzed. Different routes of administration and adjuvants were evaluated. Glycoprotein C induced a specific IgG response in serum. However, it failed to prevent entry of the virus into the lung or the appearance of clinical signs, which were variable among different treatment groups. Entry of the virus into the lung was delayed in all immunized groups. The viral titer was significantly lower when adjuvants were used, as well as in the intranasally immunized groups compared to the control group. Therefore, glycoprotein C induced partial protection against the challenge with the virus, depending on the adjuvant and the route of immunization.

Keywords : Equid alphaherpesvirus 1; glycoprotein C; immune response; BALB/c mice.

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