Abstract

TOMAS, P. A; ZIETZ, R  and  CERINO, M. C. Análisis de diversidad genética en Ziziphus mistol griseb. Mediante marcadores moleculares ISSR. FAVE. Secc. Cienc. agrar. [online]. 2017, vol.16, n.1, pp.153-162. ISSN 1666-7719.

The genetic diversity in a population of Ziziphus mistol was analysed using molecular markers. The DNA polymorphism among 31 genotypes obtained from 10 parental trees was assessed using seven inter simple sequence repeat (ISSR) primers. The analysed population showed low percentage of polymorphic bands and low genetic diversity. However, all genotypes were different in cluster analysis based on genetic distances. The molecular variance analysis (AMOVA) showed a higher proportion of variance explained within progenies. Paternal amplicons allowed to determine the cross-fertilizing origin for all progenies analysed. These results confirm the efficacy of the mechanisms favoring allogamy in this species. Possible implications of low genetic diversity in this population and the need to extend the analysis to other populations are discussed in order to elaborate and implement management strategies for conservation and use of this genetic resource.

Keywords : Ziziphus mistol; Genetic diversity; ISSR; Rhamnaceae; Genetic resources.

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