Abstract

JOLLY, A. et al. Lipoarabinomanano (LAM) de Mycobacterium spp: Respuesta inmune inducida en terneros. InVet [online]. 2006, vol.8, n.1, pp.103-109. ISSN 1668-3498.

Paratuberculosis is a chronic enteric disease affecting cattle. The causative agent is Mycobacterium avium subspecies paratuberculosis. LAM is the main antigenic component of mycobacterial surface, and it is considered a key virulence factor involved in its pathogenicity. A LAM-enriched extract obtained from a culture of Mycobacterium spp. was prepared with incomplete Freund's adjuvant for calves inoculation. Specific antibodies response and delayed-type hypersensitivity to intradermal injection of purified protein derivative antigen (PPD) from Mycobacterium bovis were then evaluated in inoculated animals. Our results demonstrate that anti-LAM antibodies can be successfully obtained in calves immunized with LAM-enriched extract, without generating cross-reaction with PPD of M. bovis. This work could represent the initial step in order to determine the relevance of LAM as a potential candidate for elaborating a subunit vaccine against bovine paratuberculosis.

Keywords : Cattle; Paratuberculosis; LAM; Immune response.

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