Abstract

TERUEL, M. T. et al. Effect of different doses of equine chorionic gonadotropin on ovary response and in vitro mouse embryo development. InVet [online]. 2006, vol.8, n.1, pp.149-156. ISSN 1668-3498.

The aim of this study was to evaluate the effect of different doses of equine gonatotropin hormone (eCG) (5, 7.5 or 10 IU) on the ovary response and in vitro embryo development in Balb C mice. The ovary weight and diameter increased for 7.5 and 10 IU of eCG compared to control group (P<0.05). The number of oocytes was higher in females stimulated with 10 IU compared with those of 5 IU of eCG (30.8 ± 12.4 and 18.9 ± 6.8 respectively, P<0.05). The number of morulae was similar between groups. The percentage of oocytes and morulae with normal morphology was not modified for the eCG level (73.8 ± 22.7; 63.8 ± 24.8; 70.6 ± 16.1 and 53.3 ± 36.0; 80.7 ± 16.0; 69.2 ± 25.5 for 5, 7.5 or 10 IU of eCG respectively, P>0.05). In vitro, the differentiation was not modified by the dose level. The hatching rate at 96 h was higher for embryos from 7.5 IU compared to 10 IU (82.22 vs 64.39, P<0.05). We concluded that, the percentage of normal oocytes and morulae and the differentiation rate are not dependent of the dose of eCG, however, the hatching in vitro is dose eCG dependent.

Keywords : Ovarian stimulation; Oocyte; Embryo development; Mouse.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )