Abstract

NAVAS, L.E; AMADIO, A.F; FUXAN, I  and  ZANDOMENI, R.O. Identificación de genes codificantes de enzimas de interés industrial en una cepa de bacteria termofílica aislada de aguas termales de Salta (Argentina). RIA. Rev. investig. agropecu. [online]. 2014, vol.40, n.1, pp.46-53. ISSN 1669-2314.

Two thermophilic bacteria were isolated from a hot spring in Salta, northwest Argentina. Phylogenic analysis indicates that the isolates belong to the Thermus and Geobacillus genera. We have undertaken the DNA sequencing of the complete genome from the isolate Thermus sp. 2.9 using Roche 454 technology. Two hundred and fifteen thousand readings were obtained providing approximately 40 fold coverage of the genome. A first round of analysis of the contigs was made to identify proteins coded in the genome. We report the identification and characterization of several genes coding for enzymes related to the degradation of polymers such as xylanases, proteases, esterases, lipases, catalase and galactosidases. These enzymes may be useful in processes to transform commodities from agriculture and valuable tools in the food industry.

Keywords : Thermophilic bacteria; Biotechnology; Thermophilic enzymes; Genomes; Industrial microbiology.

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