Abstract

TOLOCKA, Patricia A. et al. Caracterización de Xylella fastidiosa a partir de materiales vegetales y cepas aisladas de olivo (Olea europaea L.) e implementación de un sistema de diagnóstico serológico en Argentina. RIA. Rev. investig. agropecu. [online]. 2021, vol.47, n.3, pp.380-389.  Epub Dec 01, 2021. ISSN 1669-2314.

Xylella fastidiosa is considered a quarantine pest of global significance due to the severe economic and social damage it causes on most valuable crops. The objective of this work was to isolate and characterize bacterial strains of infected with X. fastidiosa of olive (Olea europaea L.) samples and implement a serological diagnostic system for their detection. For the molecular characterization, the multilocus sequence typing system (MLST) was used. The isolation of the bacterium from the olive tree was achieved and it was determined that all materials characterized correspond to X. fastidiosa subsp. pauca ST69, a genetic subgroup that has been detected only in Argentina. An antiserum was produced and serological diagnosis systems were adjusted. A solid, fast and economical diagnostic method DAS ELISA system was achieved, solving the continuous lack of availability of serological reagents for X. fastidiosa.

Keywords : xylem bacterium; MLST; antiserum; immune tests.

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