Abstract

LOPEZ-ORTEGA, A.A; ARANGUREN, A.J; PLAZA, M.A  and  MURILLO, M.D. Estrés oxidativo y alteraciones de la funcionalidad hepática en ratones hembras con hígado graso experimental. Rev. vet. [online]. 2014, vol.25, n.1, pp.7-11. ISSN 1669-6840.

The oxidative stress (OS) affects the progression and severity of different pathologies, including those affecting the liver). OS has been associated with the non-alcoholic fatty liver (NAFL), of importance in both human and veterinary medicine due to hepatic disfunction as a consequence of cellular deterioration, part of the lipid peroxidation process. The objective of this study was to determine if experimental NAFL in female NMRI mice induced by ethionine evokes OS and alters hepatic function. Fatty liver (FL) was induced by the administration of ethionine (7.5 mg/20 g body weight), of known hepatotoxic effects. Two groups of 10 animals each, were used (control and injected with DL-ethionine). FL was assessed by histology and the quantification of hepatic triglycerides, which indicated hepatic steatosis. For the determination of OS, malondialdehyde (MDA) and conjugated diene (CD) concentrations were determined by means of spectrophotometry. In addition, the concentrations of ALT and AST in plasma were quantified using commercial kits. The induction of FL caused significant elevation of liver parameters indicative of OS, MDA: from 258.30 ±22.49 nmoles/mg protein to 354.90 ±19.83 nmoles/mg protein (p<0.01), as well as DC: from 212.46 ±8.36 mmoles/mg protein to 263.93 ±14.51 mmoles/mg protein (p<0.01). In FL the plasmatic activity of aminotransferases significantly increased: ALT from 77.01 ±4.11 U/l to 198.34 ±17.22 U/l (p<0.001) and AST from 188.29 ±11.81 U/l to 401.71 ±22.42 U/l (p<0.001). It is concluded that the ethionine-induced FL is accompanied with a state of OS which compromises the liver function.

Keywords : Mouse; Fatty liver; Ethionine; Oxidative stress; Dysfunction.

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