Abstract

ECHEVERRIA, S.M. et al. Cambios microscópicos en ganglios linfáticos de ratones inoculados con veneno de Bothrops alternatus. Rev. vet. [online]. 2015, vol.26, n.2, pp.113-119. ISSN 1669-6840.

In the past, Bothrops alternatus envenoming was widely studied in many organs of mice. However, little is known about the alterations in secondary lymphoid organs. In this paper we present a preliminary histological study of the early events that occur in inguinal and popliteal lymph nodes in order to determine the pathophysiological effects that B. alternatus venom triggers in key organs of the immune system. The assay was carried out in inguinal lymph nodes of mice (male CF-1) inoculated i.v. with different doses of venom (1, 10 and 20 ug) at 24, 48, 72 and 96 h; another assay was also carried out in popliteal lymph nodes using i.m. injections of 20 ug crude venom at 6, 12, 24 and 72 h. All of the lymphoid nodes were processed according to classical techniques (H). The assessment of morphological changes was performed by microscopic examination of non-serial section cuts. In all of the cases nodes showed a cellular response without compromising tissue integrity. Early histological changes in the popliteal (6 h) and inguinal (24 h) lymph nodes were detected, characterized by subcapsular space dilatation, congestion and increase of lymphocyte migration via the high endothelial venules of the paracortex. Reactive changes were also observed in this area, with hyperplasia regardless the T cell region. No differences were detected between inguinal and popliteal lymph nodes, even for different inoculation routes. These preliminary findings highlight the deletereous effects in innate and adaptive immune system caused by B. alternatus venom besides the local tissue effects reported in previous works. Such findings in the secondary lymphoid organs contribute with updated knowledge regarding the adaptive immune responses that take place at the early stages of B. alternatus envenoming

Keywords : mice; lymph node; venom; Bothrops alternatus; histological changes.

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