Abstract

JAUREGUIBERRY, M. et al. Espectrofotometría infrarroja transformada de Fourier para identificar bacterias uterinas patógenas en vacas lecheras. Rev. vet. [online]. 2016, vol.27, n.2, pp.75-79. ISSN 1669-6840.

The objectives of the present study were to test the agreement between routine methods of bacteriological testing and the Fourier transformed infrared spectroscopy (FTIR) method for uterine bacterial identification, and if a database built with uterine bacteria from European dairy cows can be used to identify bacteria from Argentinean dairy cows. Postpartum dairy cows (n=64) with different vaginal discharge (VD) were sampled for bacterial identification. The uterine bacteria were first identified by routine bacteriological testing methods (n=27) and then by FTIR. Statistical analyses were performed by kappa's coefficient and by logistic regression analysis. The agreement between the two methods had a kappas coefficient of 0.73 (p<0.001). In addition, the likelihood for bacterial growth (E. coli and T. pyogenes) tended to increase with VD score (0.06), given that the odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD. In conclution, FTIR spectroscopy has a good agreement with routine bacteriological testing methods and the database built with bacteria from European dairy cows is useful to identify uterine bacteria from Argentinean dairy cows.

Keywords : dairy cow; uterine pathogens bacteria; infrared spectroscopy.

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