Abstract

FLORES, C. et al. Efecto antioxidante de la melatonina durante la conservación de semen de cerdo. Rev. vet. [online]. 2018, vol.29, n.1, pp.13-17. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.2912780.

During the processes of sperm preservation the free radical production increases, and it has been suggested that the addition of antioxidants improve semen quality during storage, mainly in species such as pork, where this has been linked to oxidative stress and sperm damage during storage, which affects their fertilizing capacity. The goal of this research is to determine the antioxidant effect of the melatonin during the conservation of semen of boars to 16°C. Semen samples were taken of boars 7-18 months old. Semen collection was made with gloved hand. Semen samples with optimal parameters were diluted and divided into aliquots: control (i.e., diluent), control with vehicle (diluent and ethanol) and semen with melatonin (1.25 mM). After the preparation of aliquots (day 0) and on day 1 of conservation at 16°C, lipoperoxidation indicators such as conjugated dienes (DC, isopropanol extraction) and malondialdehyde, were evaluated, The enzymatic activity of dismutase superoxide (SOD) was determined by commercial kit. The results were analyzed by ANOVA, with package statistical SPSS 15.0 for Windows with p≤0.05. Them levels of conjugate dienes (DC) in them samples with melatonin is increase to the day 0 and 1 of conservation, of way significant (p≤0,05) with regard to the control group and with the vehicle, while the concentrations of malondialdehyde to the day 1 of conservation in them samples with melatonin presented values significantly (p≤0,05) minor with respect to the control group, with greater activity of the SOD (p≤0,05). In conclusion, the addition of melatonin in dilute boar semen has an antioxidant effect during sperm storage to 16°C

Keywords : boar; conservation of semen; antioxidant; melatonin.

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