Abstract

CATUOGNO, M.S.; MONTENEGRO, M.A.  and  S.NEGRETTE, M.. Distribución de beta-catenina en lesiones displásicas de colon en ratas alimentadas con leche de búfala. Rev. vet. [online]. 2018, vol.29, n.2, pp.83-87. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.2923269.

The aim of this study was to determine the distribution of beta-catenin in cells of dysplastic lesions (DL) in rats fed with buffalo milk. Six-week Wistar male rats were administered with different milk diets during 123 and 240 days. Animals were fed with two diets: milk from buffaloes fed on natural pastures, and milk from buffaloes supplemented with fish oil containing high concentration of conjugated linoleic acid and omega-3. DL were induced by 1,2-dimethylhydrazine. Immunohistochemistry was performed to detect beta-catenin. The colonic epithelial cells clearly displayed beta-catenin membranous expression. In superficial cells, the protein could also be identified in cytoplasm with a weaker stain. In cells located deep in the mucosa it was observed a lack of protein in the cytoplasm, remaining only in the cytoplasmic membrane, thus being cytoplasm negative to the presence of beta-catenin. The enterocytes located on the muscular layer of mucosa showed a weaker membranous stain compared to superficial cell membranes. Beta-catenin in the nucleus of normal cells was not observed. In DL of buffalo milk fed rats the beta-catenin distribution substantially changed according to severity of dysplastic lesions. These findings could be useful in the detection of pre-carcinomatous lesions at early stage.

Keywords : rats; buffalo milk; dysplastic lesions; beta catenin.

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