Abstract

PULACHE, I.; ORDINOLA, A.; VIEYRA, E.  and  MENDOZA, O.. Identification of cultivable bacterial strains present in Scomber japonicus peruanus salted using a fragment of the 16S rRNA gene. Rev. vet. [online]. 2021, vol.32, n.1, pp.68-72. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.3215638.

18.

Salting fish is an ancient and economical form of preservation that is still used in developing countries such as Perú. In the Tumbes región, it is common the commercialization of mackerel (Scomberjaponicus peruanus) salted by hand, so its microbiological safety is not guaranteed. The investigation aimed to identify, by sequencing a fragment of the 16S rRNA gene, the cultivable bacteria present in salted mackerel marketed in Tumbes. From mackerel muscle tissue, bacterial strains were isolated in TSA médium supplemented with 15% NaCl, from which fragments of the 16S rRNA gene were obtained and sequenced to identify the species to which they belonged through a search in Genbank. Additionally, the moisture and NaCl contení of the salted mackerel muscle were analyzed. As a result, typical strains of salted producís were identified such as Staphylococcus sp, Halomonas sp, Staphylococcus equorum, Salinivibrio sp, Staphylococcus nepalensis, and Salinivibrio sp. However, in 70% of mackerels analyzed, there were inadequate humidity levels, as well as low levels of NaCl that indicated an inadequate salting and consequently a product of poor conservation.

Keywords : Scomberjaponicus; salted fish; genomics; bacteria; 16S ARNr gene.

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