Abstract

PORTOCARRERO, G. T. et al. Effect of resveratrol on the percentage and quality of in vitro embryos generated by separation of blastomeres in cattle. Rev. vet. [online]. 2021, vol.32, n.1, pp.101-105. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.3215644.

21.

The oxidative State is an important factor that determines the development of bovine embryos. The objective in this study was to evalúate the effect of resveratrol onthe quality of in vitro embryos generated by separation of blastomeres in cattle. Oocytes from the slaughterhouse were matured and fertilized in vitro by the conventional method. After 18 hours of fertilization, the zygotes were cultured for 3 days in synthetic oviductual fluid médium (SOF) for control and supplemented with 2 μM and 0.5 μM for the treatments. On day 3 they were stripped of their zona pellucida (ZP) to be cultivated at a rate of four blastomeres in well of the well (WOW) for 6 days in médium SOF supplemented with resveratrol. We evaluated percentage data of cleavage and embryonic división (8 to 10 blastomeres) after 3 days of culture, finding a significant difference p<0.05 with supplementation with 0.5 μM of resveratrol. At 6 days after blastomeres separation, percentage of embryos, number of total cells, live cells and dead cells were evaluated using Hoechst, FDA and PI staining respectively. There were no differences in the percentage of blastocysts between treatments; however, supplementation with 0.5 μM of resveratrol to the SOF médium had a greater amount of total cells and living cells (p<0.05). In conclusión, supplementation with resveratrol in the SOF médium does not increase the percentage of blastocysts but improves its quality using a concentration of 0.5 μM.

Keywords : clivage; embryonic development; total cells; living cells.

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