Abstract

CELY, L. D.; JARAMILLO, C. X.  and  FONSECA, M. J.. Effect o f two diluents on pregnancy rate by laparoscopic artificial insemination in sheeps. Rev. vet. [online]. 2021, vol.32, n.2, pp.221-224. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.3225747.

The aim of this study was to evaluate two commercial diluents for the freezing of sheep semen and determine their effect on the pregnancy rate when used in artificial laparoscopic insemination. The semen used was from an adult Dorper ram, whose ejaculate was divided in two and one aliquot was diluted with a commercial diluent based on soy lecithin and the other aliquot with a commercial diluent based on egg yolk. The semen was cryopreserved (100 x 106 motile sperm) and each type of dilution was used to inseminate 15 sheep. The females were synchronized with intra-vaginal sponges and inseminated via laparoscopic. The diagnosis of pregnancy by ultrasound was made 60 days after the service. The results indicated a pregnancy rate of 85% with semen diluted with soy lecithin and 78% with egg yolk, with no significant differences between treatments. Conclusions: pregnancy rate obtained with the two diluents was similar; therefore these offer the same benefits to use in ovine reproductive programs, by laparoscopic artificial insemination.

Keywords : cryopreservation; sperm; pregnancy; sheep; ultrasound.

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