Abstract

LOPEZ, A.A. et al. Variation of plasma levels of glucose and triglyceride in adult NMRI mice of both sexes with DLethionine-induced fatty liver. Rev. vet. [online]. 2022, vol.33, n.1, pp.23-28. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.3315874.

20.

The objective of the present study was to determine the plasma modifications of glucose (Gluc) and triglycerides (TG) in non-alcoholic fatty liver (NAFL) induced by DL-ethionine in male and female mice NMRI (nuclear magnetic resonance imaging). They were distributed in two groups of 10 animals of each sex: one control and the other treated with ethionine (7.5 mg/20 g body weight). After fasting for 48 hours, a blood sample with anticoagulant was obtained by coccygeal venipuncture and the plasma concentrations of Gluc and TG were quantified using a commercial kit. Euthanasia was performed under light ether anesthesia. The liver was dissected, its macroscopic appearance was evaluated and the liver homogenate was obtained, in which the concentration of TG was determined. A small portion of liver fixed in 10% buffered formalin was used for histopathological study. Statistical analysis was carried out using SPSS software program version 17.0 for Windows. In both males and females, the administration of DL-ethionine induced hepatic fat metamorphosis with lipid vacuoles in the cytoplasm of hepatocytes, as well as an increase in the concentration of triglycerides in the liver tissue (p<0.001). In females, it was observed that NAFL causes a decrease in plasma TG levels (p<0.01), a result that is in agreement with the increased glycemia they presented (p<0.05). Such effects would be due to insulin resistance, a key abnormality in NAFL. In males, the level of circulating TG was not modified, which would respond to the normal glycemia they showed. In conclusion, the induction of NAFL by the action of DL-ethionine affected the plasma parameters studied differently in males and females, perhaps due to adaptive variables to NAFL, specific to each sex.

Keywords : fatty liver; DL-ethionine; glucose; triglycerides; NMRI mice.

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