Abstract

SAPARRAT, Mario C. N.; ARAMBARRI, Angélica M.  and  BALATTI, Pedro A.. Growth and extracellular laccase production in liquid cultures of Minimidochium parvum LPSC # 548 Strain. Bol. Soc. Argent. Bot. [online]. 2007, vol.42, n.1-2, pp.39-44. ISSN 1851-2372.

Minimidochium parvum LPSC # 548, a fungus isolated from litter floating on waters of Río Santiago (Provincia de Buenos Aires, Argentina) polluted with industrial effluents and crude-oil, was grown as a shaking culture on a C-limited medium to evaluate its ability to produce extracellular laccase. The effect of anthracene, CuSO₄ · 5H₂O, ethanol, guaiacol, humic acids, Kraft lignin, MnSO₄· H₂O, Tween 20 and veratryl alcohol on its growth and extracellular laccase activity levels was also analyzed. The cultures grown on basal medium produced maximum biomass (over 420 mg/100 ml) and maximum extracellular laccase activity (351.7 ±53.3 pkat/ml) after 5 days of incubation. Among the different factors tested, only the humic acids at 0.1 % (w/v) were found to stimulate the growth of M. parvum . However, Tween 20 (0.1 %, v/v) was the only one that produced an increase of laccase activity levels up to 2.5-fold compared to the control.

Keywords : Growth; Laccase; Minimidochium parvum.

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