Abstract

CALONGA SOLIS, Verónica; BARRANDEGUY, María Eugenia  and  GARCIA, María Victoria. Divergencia histórica en Anadenanthera colubrina var. cebil (Leguminosae) analizando una región intrónica del ADN cloroplástico. Bol. Soc. Argent. Bot. [online]. 2014, vol.49, n.4, pp.547-557. ISSN 1851-2372.

Historical divergence in Anadenanthera colubrina var. cebil (Leguminosae) analyzing an intronic region of chloroplast DNA. Anadenanthera colubrina var. cebil is a native woody tree from South America which presents a discontinuous distribution which is similar to that observed for Seasonally Dry Tropical Forests (SDTFs). To evaluate the probable effects of climate changes during Pleistocene in A. colubrina var cebil distribution, the chloroplast trnL intron sequence of 48 individuals from Paranaense and Yungas phytogeographic provinces were analyzed. A SNP in the position 501 defined two haplotypes. These haplotypes were fixed according to phytogeographic provinces of individual's origin. The sequences from the trnL intron of these haplotypes were compared with sequences from the same region available in GenBank public database. Three more haplotypes were defined based on the alignment analyses of these sequences. A network was constructed to determine the phylogenetic relationships between haplotypes. The estimation of the genetic distance between haplotypes reached values among 0 and 0.017, while the divergence time between haplotypes was estimated among 0 and 5,600,000 years.

Keywords : Anadenanthera colubrina var. cebil; Historical climate changes; Chloroplast DNA.

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