Abstract

PERERA, M. Francisca et al. Botanical characterization of new sugarcane cultivars through molecular and phenotypical markers. Rev. ind. agric. Tucumán [online]. 2020, vol.97, n.2, pp.5-9. ISSN 1851-3018.

The Sugarcane Breeding Program of Agroindustrial Experimental Station Obispo Colombres continuously generates new varieties of sugarcane, especially for the province of Tucumán, Argentina. Accurate varietal identification is essential to protect the intellectual property rights of new varieties. In this work, Target Region Amplified Polymorphism (TRAP) and Simple Sequence Repeat (SSR) molecular markers were used, in combination with phenotypic markers proposed by the International Union for Plant Variety Protection (UPOV), to characterize three sugarcane varieties recently released by the local breeding program. Five combinations of TRAP primers and five pairs of SSR primers were used. The amplification products were separated by electrophoresis in polyacrylamide gels under denaturing conditions. For the phenotypic characterization, the nine mandatory UPOV descriptors were used. The bands and morphological data were transformed into binary matrixes of 0 and 1, the similarity was calculated and the dendrograms were generated by the Unweighted pair-group method using arithmetic averages (UPGMA) analysis. Both molecular markers clearly revealed the phylogenetic relationship among genotypes. The morphological characterization correctly differentiated the varieties analyzed; only revealing the external similarity between genotypes. The entire characterization allowed us to unambiguously differentiate the recently released cultivars to register and protect plant breeders rights. 

Keywords : SSR; TRAP; UPOV´s descriptors.

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