Abstract

DIAZ, María Elena et al. Production process of sugarcane vitroplants of guaranteed genetic purity and health in the laboratory stage at the EEAOC. Rev. ind. agric. Tucumán [online]. 2020, vol.97, n.2, pp.39-44. ISSN 1851-3018.

The Vitroplantas Project of sugarcane of Estación Experimental Agroindustrial Obispo Colombres (EEAOC) was started in 2001. Its main goal is to provide sugarcane producers with seed cane of high phytosanitary quality and guaran-teed genetic purity. Since its beginning, improvements in the process such as molecular techniques for disease diagnosis and assessment of genetic purity, have been incorporated. Until 2004 the vitroplant sanitary evaluation for two systemic diseases was carried out by serological techniques. However, since 2005, molecular diagnoses based on PCR for the most regionally important systemic diseases of sugarcane were incorporated. In 2014, the genetic identity analysis of vitroplants undertaken since 2007 with AFLP was replaced by TRAP since it was cheaper and revealed similar polymorphisms. Recently, a Quality Management System, which allows effective administration and increases the efficiency at each step of the micropropagation process, was implemented. Over the 2001-2019 period, 13 commercial and promising sugarcane clones were micropropagated, reaching a total of 1,210,519 Vitroplants. Currently, around 70% of the area under cultivation in Tucumán is occupied by high quality sugarcane seeds produced by the Vitroplantas Project, resulting in lower disease incidence and a gradual increase in yields in commercial fields. This Project has constantly looked at improving the process to obtain a product able to satisfy sugarcane producersdemands.

Keywords : hydrotermotherapy; molecular diagnosis; molecular markers; tissue culture.

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