Abstract

CASTANEDA-GARZON, S. L.  and  PEREZ-MARTINEZ, B. A.. Germinating Drimys granadensis L.f. (winteraceae) in greenhouse and in vitro laboratory conditions. Quebracho (Santiago del Estero) [online]. 2017, vol.25, n.1, pp.40-53. ISSN 0328-0543.

The need for strengthening ex situ native medicinal species conservation processes has led to the continued development of studies for the identification of plant breeding protocols. Recently, the Drimys granadensis L.f. (canelo de páramo) species was included in sexual propagation studies, to evaluate its germination when using pregerminative treatments. The process developed by identifying and selecting the candidates for seed sources, the collection and preparation of plant material, its establishment, monitoring and evaluation of the germination tests. The maximum germination rate in greenhouse (63.50 %) was obtained by stratifying the seeds at 4 °C (river sand and hydration) and seeding in black vermicompost (50-50 %) soil substrate under a shade percentage of 30 %. Applying gibberellin A3 at concentrations of 1000 ppm and 2000 ppm, is equally favorable when sown directly in peat and/or river sand (100 %). For in vitro germination, the highest level of disinfection was obtained using sodium hypochloride at 5,25 % with explant immersion for 5 minutes and germination is influenced by the removal of the seed coat and planting in a culture medium with neither growth regulators nor organic supplements.

Keywords : Colombia; High Andean forest; Culture medium; Native species; Sexual propagation.

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