Abstract

L. M., Klein; SPOLJARIC, M. V.  and  TORALES, S. L.. Identifying stable genotypes in 19 families of Prosopis alba using microsatellite markers and productivity parameters. Quebracho (Santiago del Estero) [online]. 2019, vol.27, n.1, pp.26-26.  Epub Mar 01, 2019. ISSN 0328-0543.

Nineteen (19) white carob families from Rio Bermejito (General Güemes Department in the Province of Chaco) belonging to the field collection of Prosopis established in 2010 were selected from the INTA-Saenz Peña germplasm bank due to their genetic stability and characterized by molecular and morphometric markers. This work was conducted in the Agricultural Experimental Station (EEA in Spanish) of the National Institute of Agricultural Technology (INTA in Spanish) located in Sáenz Peña (Latitude South 26º47'27" and West Length 60º26'29", Altitude 90 m s.n.m.), Chaco during 2014-2016. The molecular characterization was performed using fourteen microsatellite markers (SSR) while the morphological one was carried out by analyzing different morphometric variables. When studying the genetic similarity among the 19 families, the progenies were related into a single group showing 45 % of similarity without being grouped by family. The study of genetic diversity revealed similar frequency between families. Furthermore, the expected value for heterozygosity (He) was lower than the observed (Ho) which indicates inbreeding. The AMOVA and the Wright’s F statistical showed that the greatest genetic differentiation occurs within each family and it is lower among families. There is a 4 x 4 distancing in the plantation with four (4) pseudo-repetitions per family. The descriptive statistical analysis together with the nonparametric Kruskal Wallis at 5 % of variance were both applied; both showed non-significant differences among families, being total height the most stable parameter. This study will allow a first step in the selection of stable genotypes for conventional breeding improvement be taken.

Keywords : SSR; morphometrics; white carob.

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