Abstract

GONZALEZ, MD; LUIS, CM  and  LANZELOTTI, PL. Polyphenolic profile of Origanum vulgare L. ssp. viridulum from Argentina. Phyton (B. Aires) [online]. 2014, vol.83, n.1, pp.179-184. ISSN 1851-5657.

Characterization of oregano in Argentina is limited. They are normally known with fancy names, but it is not possible to recognize whether they are genetically equivalent or not. This paper presents a non-volatile polyphenol study of one of the subspecies of Origanum vulgare, O. vulgare L. ssp. viridulum (Martrin-Donos) Nyman [= O. vulgare L. ssp. virens (Hoffmannsegg et Link) Ietswart]. The polyphenols 3-(3,4-dihydroxyphenyl)lactic acid, 3,4-dihydroxybenzoic acid, caffeic acid, 4-(3.4-dihydroxybenzoyloxymethyl)phenyl β-glucoside and rosmarinic acid were isolated and identified by spectroscopic analysis from leaves and inflorescences of classified material. In addition, the polyphenol profile of the hydroalcoholic extract of this oregano was developed applying high performance liquid chromatography (HPLC) with diode array detector (DAD), and the identified compounds were located in the profile. The observation of the instrumental UV spectra revealed that this subspecies is also rich in flavone derivatives such as apigenin and luteolin glycosides. The profile found appears repeatedly in other samples of the same variety with diferent geographic origins, while differences were observed in profiles of other taxonomical groups of oreganos. This chemical profile is expected to be an additional support for botanists who diferentiate origanum materials, especially when most of the plant features have been lost in the culinary herb.

Keywords : Origanum vulgare L. ssp. viridulum; Polyphenols; Phenyl glucosides; Flavone glycosides; HPLC-DAD profile.

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