Abstract

HERMOSILLO-CERECERES, MA et al. Impact of selenium fertilization on the activity of detoxifying enzymes of H₂O₂ in bean plants. Phyton (B. Aires) [online]. 2014, vol.83, n.2, pp.347-352. ISSN 1851-5657.

Selenium (Se) is an essential element for many organisms, although in high concentrations it may become toxic, leading to the generation of reactive oxygen species (ROS). In this study, bean plants received different application rates of Se (0, 10, 20, 40, 80, and 160 μM) in the form of selenite and selenate to evaluate the activity of the detoxifying enzymes of H₂O₂. The results indicated that the activity of these enzymes in bean plants depended on the chemical form of Se: selenite at rates of 20 μM or greater diminished biomass and yield, increasing the activity of superoxide dismutase (SOD). Even when catalase (CAT) activity also increased, it appeared that it was efcient at detoxifying H₂O₂ in the presence of Se, given also the rise in H₂O₂ production. Also, selenate diminished yield up to a rate of 160 μM and increased the activity of the enzyme GSH-Px, which reached its maximum activity at 160 μM, and thus proved less toxic than selenite.

Keywords : Phaseolus vulgaris L; Green bean; Biofortification; Selenium.

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