Abstract

PARAVANI, Enrique V.; ACOSTA, María G.; DIAZ ZAMBONI, Javier E.  and  CASCO, Víctor H.. Isolation, cloning and expression of the recombinant protein for the fibroblast growth factor 8 (FgF-8) of Rhinella (=Bufo) arenarum. Rev. cienc. tecnol. [online]. 2009, n.12, pp.17-22. ISSN 1851-7587.

Embryological studies have demonstrated that the origin of organs in vertebrates involves complex cellular interactions in different tissues. Biochemical and molecular evidence suggests that endogenous signal in the apical ectodermical ridge (AER) are exerted by Fibroblastic Growth Factor 8 (FGF-8). This Factor is essencial in the initiation of the limb development processes in vertebrates. The main objective of the present work was to use molecular techniques to obtain a recombinant protein to FGF-8, from the isolation of the Rhinella arenarum toad gene, and to analyze their nucleotidic sequence using bioinformatic tools. It was possible to express the recombinant FGF-8 in a soluble form and also to study the evolutionary relationship with different vertebrate species. The results reflect a near evolutionary relation with other homologous vertebrates genes, especially with the amphibians. The obtencion of the recombinant protein gave us the possibility of carrying out structural and physiologycal studies about the role of FGF-8 during the limb development in our experimental animal model (R. arenarum). A secondary objective was to produce specific polyclonal antibodies which allowed for the search of other possible locations and roles of this factor during vertebrate development.

Keywords : Fibroblast Growth Factor 8 (FGF-8); Recombinant protein production; bioinformatics tools.

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