Abstract

RIERA, Mario Alejandro; ROJAS, María Eugenia  and  ZAPATA, Pedro Darío. A modified salting-out protocol for DNA extraction from small blood sample volumes. Rev. cienc. tecnol. [online]. 2010, n.14, pp.4-7. ISSN 1851-7587.

A modified salting-out procedure for DNA extraction from decremental blood sample volumes was developed (300; 100; 50; 25; 10; 5; 2,5; 1 and 0,5 µL). A 345 bp human prothrombin gene fragment and a STR marker commonly used for filiation analyses (LPL) were then amplified by Polymerase Chain Reaction and visualized in a 6.4 % polyacrylamide gel. In all cases the amplification reaction was successful, showing that the DNA extraction method proposed here yields DNA of reasonable purity and quantity from small blood sample volumes. Therefore, the quantity of sample needed to perform a molecular study can be reduced, which is fundamental in certain areas such as forensic genetics, where the quantity of samples available could be limited.

Keywords : Blood sample; DNA extraction; salting-out; PCR.

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