Abstract

ORTELLADO, Laura E et al. Effect of metal ions and aromatic compounds on laccase activity in Trametes sp. native of Misiones. Rev. cienc. tecnol. [online]. 2016, n.26, pp.4-10. ISSN 1851-7587.

The white rot fungi secrete oxidative enzymes, such as laccase, with a capacity to play a part in environmentally friendly processes such as bioremediation. The aim of this study was to develop a culture medium supplemented with aromatic compounds and metal ions in order to increase laccase activity produced by Trametes sp. strain A. By means of a Plackett-Burman design, it was shown that Mn2 + was the only variable that had a significant positive effect on the enzyme activity. The highest laccase activity (272.46 U / L) was obtained at a concentration of 1.5 mM Mn2 + and maximum biomass (0.1272 g) at a concentration of 4 mM Mn2 +. It also was possible to establish a negative correlation between enzyme activity and growth of fungus. An isoenzyme of constitutive expression (60 kDa) and another inducible (100 kDa) were found in the presence of 2; 2.5; 3; 3.5 and 4 mM of Mn2+.

Keywords : Laccase activity; White-rot fungi; Trametes; Isozymes.

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