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Revista de Ciencia y Tecnología
On-line version ISSN 1851-7587
Abstract
PRIGIONI, Gaston Ariel; RODRIGUEZ, Maria Daniela; ZAPATA, Pedro Darío and VILLALBA, Laura Lidia. Corn liquor as a culture medium for laccase production by Phlebia brevispora. Rev. cienc. tecnol. [online]. 2018, n.30, pp.1-12. ISSN 1851-7587.
The objective of this work was to test different culture media in order to achieve high laccase production by Phlebia brevispora using low cost substrates. With a medium composed of malt extract, corn liquor and CuSO4, 3.807 U/L of laccase at day 14 of incubation were obtained. Laccase production was tested with two different soluble corn extracts, analytical and industrial grade, but no statistical significant differences in laccase production were detected when using corn liquor of industrial (3,761U/L), or analytical grade (3,726U/L), both values attained at day 10 of incubation. Thus, an inexpensive medium for laccase production could be selected, also replicable in a 5 L bioreactor.
Keywords : Phlebia brevispora; Laccase; Production; Corn liquor; Bioreactor.