Abstract

BARUA, Ramona C. et al. Isolation and identification of amylolytic and cyanide tolerants microorganisms from cassava starch industry effluents. Rev. cienc. tecnol. [online]. 2021, n.35, pp.1-10. ISSN 1851-7587.

Cassava cultivation in the Misiones province largely contributes to the national production. Part of it is used to obtain starch, which generates industrial effluents with a high load of organic matter and cyanogenic compounds. This constitutes a problem at the environmental level and bioremediation is presented as an alternative to reduce this waste, since it is based on the degradation of the pollutant using microorganisms present in the contaminated matrix. The objective of this work was to isolate microorganisms with starch degradation capacity and tolerance to cyanide from effluents of the starch industry. We isolated 93 bacterial strains and 15 were preselected by qualitative determination of amylolytic activity. Using the 16S rDNA gene for molecular identification, it was determined that some of these isolates belong to the genera Pseudomonas, Acinetobacter and Proteus. These strains were cultivated in minimal medium with different cyanide concentrations and it was possible to determine that one of them has amylolytic activity and tolerance to cyanide, both desirable characteristics for bioremediation of effluents from the cassava starch industry.

Keywords : Cassava; Wastewater; Bacteria; Starch; Cyanide; Bioremediation.