Abstract

CELAYA, Liliana,; FERREYRA, Darío,; KOLB, Eugenio,  and  KOSLOBSKY, Nicolás, Kolb. Extraction of Stevioside and Rebaudioside A from Stevia rebaudiana by percolation with ethanol-water solvents. Rev. cienc. tecnol. [online]. 2022, n.38, pp.61-70. ISSN 1851-7587.  http://dx.doi.org/10.36995/j.recyt.2022.38.007.

The aim of this study was to analyze the optimum temperature and percentage of ethanol to extract steviol glycosides (Stevioside and Rebaudioside A) from stevia leaves in a percolation extractor. The experiments were performed in broad ranges of binary solvent (0-100 % ethanol) and temperature (30-70 °C) in order to investigate their effects on steviol glycosides recovery and purity. The optimal extraction conditions, which gave a best steviol glycosides extraction kinetic were 70 °C and 35% of ethanol. In addition, the optimal extraction condition giving maximum purity was 70% of ethanol between 30 and 70°C.

Keywords : Steviol Glycosides; Crude Extract; Percolation; Ethanol Water; Purity; Yield.

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