Abstract

MONETTI, Marina et al. The presence of periodontopathogens associated with the tumour necrosis factor-a expression in patients with different periodontal status. Acta odontol. latinoam. [online]. 2012, vol.25, n.1, pp.82-88. ISSN 1852-4834.

The purpose of this study was to investigate the relationship between P. gingivalis, T. forsythia, T. denticola, P. intermedia, and A. actinomycetemcomitans in the sulci or pockets of patients with gingivitis (G), mild chronic periodontitis (MiCP), moderate chronic periodontitis (MoCP) and severe periodontitis (SP), and the expression of TNF-á in gingival tissue associated with clinical parameters. Six patients with G, 7 with MiCP, 23 with MoCP and 7 with SP were recruited. Pathogens obtained from the sulci or pockets were identified by PCR, and expression of TNF-á from gingival tissue was analysed. Probing depth (PD), clinical attachment level (CAL) and loss of bone were recorded. P. gingivalis was detected at the following rates: 16.6% in subjects with G, 57.1% in MiCP, 57.8 % in MoCP and 58.1% in SP (p<0.05). P. intermedia was not identified in subjects with G. A. actinomycetemcomitans was only identified in subjects with MoCP (31.5%) and SP (42.8 %). T. denticola and T. forsythia were identified in all subject groups. Bacterial combinations were identified as follows: P. denticola + P. intermedia and P. intermedia + T. forsythia were associated (p = 0.04, p = 0.02) with the presence of TNF-á mRNA in 20% and 25% of subjects, respectively. P. gingivalis + A. Actinomycetemcomitans and A. actinomycetemcomitans + T. forsythia were associated with severe PD and CAL, respectively. The association between the presence of P. intermedia and expression levels of TNF-á was significant (p = 0.05). These results indicate that the proportion of patients with P. gingivalis increases with the progression of disease. We observed that the presence of P. intermedia may trigger the expression of TNF- á and cause a worsening of the patient's clinical status.

Keywords : Periodontal diseases; Microbiology; Tumor Necrosis Factor alpha; Periodontal index.

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