Abstract

REIGOSA, M.; LABARTA, V.; MOLINARI, G.  and  BERNALES, D.. Cytocompatibility, cytotoxicity and genotoxicity analyses of dental implants. BAG, J. basic appl. genet. [online]. 2008, vol.19, n.1, pp.43-48. ISSN 1852-6233.

Different materials are used for prostheses in dental medicine, though titanium is the most frequent. In the present report, several commercial dental implants and their potential capacity to integrate to bone tissues have been analysed by means of cytotoxicity, genotoxicity and cytocompatibility techniques. Cultures from osteosarcoma-derived UMR 106 cell line were used for bioassays since they showed several osteoblast properties. Samples of dental implants provided by B&M national company (ASTM titanium 8348 GR2, acid-etched surface) were compared with some others commercialized by foreign firms in Argentina (Nobel: anodic-oxidized surface, and 3i: sandblasted surface). Using the neutral red uptake test, lysosome and alkaline phosphatase enzymatic activities were measured in order to assess cytotoxicity level. Acridine ethidium/ orange bromide technique was used to determine cell viability, and genotoxicity was analysed using the comet assay. Results from bioassays showed high biocompatibility and no significant differences among dental samples evaluated. Techniques employed for the present study proved to be a useful tool for the analysis of other materials used in dental medicine.

Keywords : Cytotoxicity; Cytocompatibility; Single cell electrophoresis; Osteoblast.

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