Abstract

RANDAZZO, C.P; ROSSO, B.S  and  PAGANO, E.M. Identificación de cultivares de trébol blanco (Trifolium repens L.) mediante SSR. BAG, J. basic appl. genet. [online]. 2013, vol.24, n.1, pp.19-26. ISSN 1852-6233.

White clover (Trifolium repens L.) is a forage legume of high nutritive value. It is a very efficient species for nitrogen soil fixation through symbiosis. In Argentina, it has widely spread in humid and sub-humid regions. The aim of this work was to evaluate SSR molecular markers for genetic differentiation and identification of white clover cultivars. Relationships among cultivars were established and cultivars were grouped on the basis of molecular markers and morpho-agronomic characters . Twenty- four white clover cultivars and two red clover cultivars from different origins were evaluated. Fifteen SSR fluorescence markers were employed. PCR products were separated using ABI PRISM 3100 genetic analyzer. A total of 114 amplification products were obtained, with 99 % polymorphism. Average number of bands per SSR was 8.66. Cultivar similarity ranged from 15 % to 85 % , being Haifa and Zapicán the most similar. Cultivars from New Zealand were genetically more distant, with similarity values lower than 50 %. Correlation between morphological and genetic matrixes was significant (r= 0.48). All cultivars clustered in three groups, following a pattern which corresponded mainly to white clover leaflet type.

Keywords : Molecular markers; Microsatellites; White clover; Cultivars.

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