Abstract

CHIAPERO, Ana Laura; LAS PENAS, María Laura; AMELA GARCIA, María Teresa  and  BERNARDELLO, Gabriel. Estudios citogenéticos en especies de Passiflora subgénero Passiflora (Passifloraceae). Bol. Soc. Argent. Bot. [online]. 2013, vol.48, n.1, pp.103-110. ISSN 1851-2372.

Passiflora has nearly 525 spp. with x = 9 or 6. A cytogenetic study of four species from subgenus Passiflora that grow in Argentina was performed. Giemsa technique, fluorescent chromosome banding CMA/DAPI and FISH (probe 18-5,8-26S and 5S) were used in mitotic chromosomes. P. caerulea, P. tucumanensis and P. cincinnata resulted diploid (2n = 18) while P. mooreana tetraploid (2n = 36). The karyotypes were highly symmetrical, with the formula 8 m+ 1 sm, duplicated in P. mooreana. With CMA/DAPI, diploids showed 2-3 pairs of terminal bands CMA + /DAPI - , whereas the tetraploid five bands, associated to NOR in all cases. With FISH, the loci 18-5.8-26S coincided in number and position with the CMA + /DAPI - bands. In each species, the number of sites for 5S rDNA genes agreed with the ploidy level. Data of P. tucumanensis and P. mooreana are here reported for the first time, being this last species the first known tetraploid for subgenus Passiflora. FISH patterns and heterochromatic bands varied among the Passiflora species studied, mainly with respect to the number and distribution of the signals.

Keywords : Passiflora; Karyotypes; Heterochromatin; DNAr 18-5.8-26S and 5S.

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