Abstract

MUNOZ, Mario; MOLINE, Martín  and  LIBKIND, Diego. Comparación de técnicas para el aislamiento y recuento de levaduras y hongos dimórficos del filoplano de nothofagus pumilio. Bol. Soc. Argent. Bot. [online]. 2013, vol.48, n.2. ISSN 1851-2372.

Comparison of techniques for isolation and enumeration of yeast and yeast like fungi from Nothofagus pumilio phylloplane. The phylloplane is an important habitat for microorganisms. This study aimed to test different methods for the isolation of yeasts and dimorphic fungi from the phylloplane of Nothofagus pumilio, the dominant tree species in Patagonia. The tested methods included printing on agar, vertical agitation on water, with the addition of surfactant compounds (Tween 80) or abrasive material (sand), maceration and ultrasound treatment. A method that combines the vertical agitation, with application of ultrasound was established as the most appropriated for the isolation and enumeration. With this method it was determined that the density of yeasts and dimorphic fungi in the phylloplane of N. pumilio ranged between 10 and 10³ colony forming units per cm², values between 10 and 100 times lower than those observed for other tree species. Also, the dimorphic fungi Aureobasidium pullulans was determined as the dominant fungal species in this environment.

Keywords : Aureobasidium; High altitudes; Lenga; yeasts; Patagonia.

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