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Acta bioquímica clínica latinoamericana
versión impresa ISSN 0325-2957versión On-line ISSN 1851-6114
Resumen
VALERO CHAVEZ, Francisco Javier et al. Comparative study of the determination of vitamin D by two immunoassays. Acta bioquím. clín. latinoam. [online]. 2017, vol.51, n.4, pp.593-601. ISSN 0325-2957.
Currently, Vitamin D is a highly important metabolite as a result of the discovery of its new functions. The best way to measure the levels of vitamin D in the body is by measuring the levels of 25(OH)vitamin D, but currently there is no standardization between methods and therefore there is a great variability between them. In clinical laboratories, immunoassays are the most used methods, and only ADVIA Centaur Vitamin D Total assay© and IDS-iSYS 25-Hydroxy Vitamin D©passed the first phase of the VDSCP designed by the CDC. In this research study, these 2 immunoassays were compared, and although both passed the first phase of the VDSCP, the results showed that there still exists variability between methods. Therefore, they are not interchangeable, being it necessary to define some reference values for each method until the standardization method is finishe, and there is a good correlation between methods.
Palabras clave : Vitamina D; Immunoassay; Comparison; Interferences; Variability.