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Revista argentina de microbiología

versión impresa ISSN 0325-7541versión On-line ISSN 1851-7617

Resumen

RICANO RODRIGUEZ, Jorge; RICANO RODRIGUEZ, Celeste; YONG, Daniela Luis  y  GUZMAN LOPEZ, Oswaldo. First evidence of nitrilase enzymatic activity of Xylaria sp. and its relationship with the biosynthesis of indole-3-acetic acid. Rev. argent. microbiol. [online]. 2023, vol.55, n.3, pp.4-4. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2023.01.008.

Endophytic fungi inhabit plant tissues internally and asymptomatically, and many of them are involved in the synthesis of bioactive metabolites of antifungal and therapeutic nature, as well as other compounds of biotechnological importance including indole derivatives, among many others. Ecologically, they provide some benefits to plants including protection against phy-topathogens and promotion of root growth. In this sense, Xylaria sp. is a cellulose-decomposing fungus with biotechnological potential. It is worth mentioning that indole-3-acetic acid (IAA) also plays an extremely important role in plant-micro-organism interactions, as it is essential for physiology and proper plant morphological development. It is known that nitrile-hydrolytic enzymes (nitrilases) are involved in the synthesis of plant indole compounds; however, relatively little information is available concerning the nature of these enzymes in the fungal kingdom. In view of the above, through a biochemical and molecular-genetic approach, it has been demon-strated for the first time that Xylaria sp. carries out nitrile-hydrolytic enzyme activity using nitrogen and carbonrich compounds as substrate. The studied strain increased its relative gene expression levels and showed mycelial growth, both in the presence of chemical compounds such as cyanobenzene and KCN. Thus, the results of this work suggest that the micro-organism is capable of degrading complex nitrogenous molecules. On the other hand, through fungal biofertilization, it was observed that Xylaria sp. promotes the development of the root system of Arabidopsis thaliana seedlings, in addition to synthesizing IAA.

Palabras clave : Indole-3-acetic acid; Nitrogenous compounds; Lignocellulolytic fungi; Nitrilases; Xylaria sp.

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